Bio LabIn operation

A real lab, in a real building.

Four stations for plant tissue culture: sterilization, laminar flow, incubation, grow-out. We run micropropagation lines, work on engineered pigment, and photograph every jar.

The lab — wide shot of all four stations — 21:9

The stationsIn sequence
01

Prep and sterilization

Media formulation, decanting, and autoclaving. Everything that enters a jar passes through here first.

TR250E 14 L autoclave
02

Laminar flow

All transfers and subcultures. HEPA-filtered laminar airflow keeps the working field sterile.

HEPA laminar flow hood
03

Incubation

Temperature and photoperiod control for culture initiation and multiplication.

VIVOHOME incubator
04

Grow-out

Acclimatization from jar to soil — the stage where most micropropagation losses happen.

Grow chamber, humidity-staged

Autoclave — real photo — 4:3

Flow hood, working field — 4:3

Jars in the incubator — 4:3

Current researchBlue cotton

Cotton that grows its own blue.

Denim is dyed with synthetic indigo, and the dyeing is the dirtiest step in the supply chain. We are working on cotton that makes a blue pigment in the fibre itself — expressing the bacterial bpsA and sfp genes in the Coker 312 line, driven by fibre-specific promoters.

Validation runs in tobacco first, because it is fast and cheap to be wrong in. Freedom-to-operate has been reviewed and the relevant promoter patents appear to have expired.

Coker 312 explants in culture — 4:3

bpsAsfpCoker 312Fibre-specific promoterTobacco validation
The missionHiganbana

Blue cotton is the R&D.
The blue spider lily is the mission.

Lycoris radiata — the higanbana — blooms scarlet along Japanese graveyards and rice-paddy borders at the autumn equinox, on a bare stem with no leaves. It has been a marker for the boundary between worlds for centuries.

A blue one has never existed. The genus has no pathway to the pigment, so no amount of breeding will produce it. That is exactly why it is worth aiming at: it is a clear, honest, very hard target, and everything we learn getting there is useful somewhere else first.

Built on higanbana folklore, which is public domain and considerably older than anything it has since appeared in.

Open datasetIn collection

A labeled image dataset for micropropagation.

Every jar is photographed on a fixed rig at every subculture and once a week between, paired with a record of medium, growth regulators, contamination, and outcome at transfer. Contamination detection and vigour scoring in tissue culture are still done by eye. There is no public dataset for it. We are building one.

Fixed rigWeekly captureContamination labelsVigour scoringOpen release

Capture rig — jar on the copy stand — 4:3

EquipmentSpecification
Autoclave
TR250E, 14 L chamber
Media and instrument sterilization
Laminar flow hood
HEPA-filtered, horizontal
Sterile transfer field
Incubator
VIVOHOME, temperature controlled
Initiation and multiplication
Grow chamber
Humidity-staged, LED photoperiod
Acclimatization to soil
Orbital shaker
Variable speed
Liquid culture and suspension work